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LRP1 expression enhances ACE2-mediated internalization of SARS CoV-2 pseudovirions. HEK293 cells stably expressing ACE2 were transfected with GFP-LRP1, and transfection was confirmed by immunoblot analysis ( A ). B – D , LRP1-transfected cells were incubated with pseudovirions containing Vesicular Stomatitis Virus (VSV) ( B ), SARS-CoV-2 S-protein ( C ) or Δenv pseudovirions ( D ). (Students t test; ∗ p < 0.05, ∗∗∗∗ p < 0.0001).

Journal: The Journal of Biological Chemistry

Article Title: The LDL receptor-related protein 1 (LRP1) facilitates ACE2-mediated endocytosis of SARS-CoV2 spike protein-containing pseudovirions

doi: 10.1016/j.jbc.2025.110227

Figure Lengend Snippet: LRP1 expression enhances ACE2-mediated internalization of SARS CoV-2 pseudovirions. HEK293 cells stably expressing ACE2 were transfected with GFP-LRP1, and transfection was confirmed by immunoblot analysis ( A ). B – D , LRP1-transfected cells were incubated with pseudovirions containing Vesicular Stomatitis Virus (VSV) ( B ), SARS-CoV-2 S-protein ( C ) or Δenv pseudovirions ( D ). (Students t test; ∗ p < 0.05, ∗∗∗∗ p < 0.0001).

Article Snippet: The SARS-CoV-2 spike protein receptor binding domain (his tagged, containing residues Arg319-Phe541) was purchased from R&D systems (Catalog number 10523-CV-100).

Techniques: Expressing, Stable Transfection, Transfection, Western Blot, Incubation, Virus